Xuning

   Xu Ning, female, associate professor of School of Pharmacy of Zhejiang University of Technology, master's supervisor, visiting scholar of Massachusetts Institute of Technology, member of Jiusan Society. I graduated with a doctoral degree in Infectious Diseases from the Key Laboratory of Infectious Disease Diagnosis and Treatment at Zhejiang University. Postdoctoral fellow in Agricultural Machinery, School of Food Science and Engineering, Zhejiang University. Committed to research on the application of near-infrared and Raman spectroscopy imaging in the pharmaceutical field. Led and completed more than ten projects, including the National Natural Science Foundation of China and the Provincial Natural Science Foundation of China. Published over 20 high-level SCI papers in the first district of the Chinese Academy of Sciences, including Analytical Chemistry, Journal of Medical Chemistry, Sensors and Actors B: Chemical.

   Lectured courses such as Cell Biology, Cell and Molecular Biology (bilingual), Research Methods of Pharmaceutical Chemical Biology (bilingual), Innovation Engineering Practice, Innovation and Entrepreneurship Planning and Practice, and hosted and mainly participated in six educational reform projects. National and provincial excellent instructors of Internet plus innovation and entrepreneurship, guiding students to win the 2020 gold medal and 2021 silver medal of the main track creative group of the China International Internet plus College Students Innovation and Entrepreneurship Competition; Gold medal and third place winner of Internet plus College Students Innovation and Entrepreneurship Competition in Zhejiang Province.

1. Phenotype Identification of HeLa Cells Knockout 

CDK6 Gene Based on Label-Free Raman Imaging.

Analytical Chemistry (IF 8.008) 

Pub Date : 2022-06-15 ,DOI:10.1021/acs.analchem.2c00188

论文下载处.pdf 

2. Novel 1,3,4-Selenadiazole-Containing Kidney-Type Glutaminase Inhibitors Showed Improved Cellular Uptake and Antitumor Activity

Journal of Medicinal Chemistry (IF 8.039) 

Pub Date : 2018-12-13 ,DOI:10.1021/acs.jmedchem.8b0119

论文下载处.pdf

3. Label-free Raman spectroscopy monitoring of cytotoxic response induced by a telomerase inhibitor.

Sensors and Actuators B: Chemical (IF 9.221) 

Pub Date : 2019-04-21 ,DOI:10.1016/j.snb.2019.03.146

论文下载处.pdf

Be the speaker:

Cell Biology, Cell and Molecular Biology (Bilingual), Research Methods in Pharmaceutical Chemical Biology (Bilingual), Innovation Engineering Practice, Innovation and Entrepreneurship Planning and Practice

Undertaking teaching construction and teaching research and reform projects:

1. The combination of combinatorial chemistry and high-throughput screening technology accelerates the discovery of sorafenib, chair, 2021.5-2022.5

2. Exploration on the cultivation mode of pharmaceutical professional degree graduate students in the context of mass entrepreneurship and innovation competition - practice of research practice competition innovation ability cultivation, hosted, September 2021.5

3. Development of Virtual Simulation Experiment on the Application of Cell Culture Technology in Drug Screening, Part 3, 2018-2020

4. Virtual Simulation Experiment Design and Script Construction for MTT Method Antitumor Drug Screening, Hosted, 2018-2019

5. Reform and Practice of Hybrid Teaching Mode in Cell and Molecular Biology, Hosted, 2017-2019

Outstanding Undergraduate Guidance Teacher at the School Level in 2021

Candidate for the 2021 School Education Award

Outstanding Instructor of Zhejiang International Internet plus Undergraduate Innovation and Entrepreneurship Competition in 2021

Outstanding Innovation and Entrepreneurship Instructor at the 2020 China International Internet Plus College Student Innovation and Entrepreneurship Competition

In 2012, Jiusan Society was an excellent member at the grass-roots level directly under the province

Instruct students to participate in the Internet plus Undergraduate Innovation and Entrepreneurship Competition:

Silver Award of Undergraduate Creative Group at the Main Track of the 7th China International Internet plus Innovation and Entrepreneurship Competition in 2021

In 2021, the 7th Zhejiang International Internet plus Innovation and Entrepreneurship Competition won the gold medal of the undergraduate creative group of the main track in Zhejiang Province

In 2020, the 6th China International Internet plus Undergraduate Innovation and Entrepreneurship Competition Main Track Graduate Creative Group Gold Award

In 2020, the 6th Zhejiang International Internet plus Undergraduate Innovation and Entrepreneurship Competition won the third place and gold medal

2019 The fifth Internet plus Innovation and Entrepreneurship Competition in Zhejiang Province

Guide students to participate in other extracurricular technology activities:

2022 Zhejiang Province Challenge Cup Gold Award

2021 China (Pan'an) Traditional Chinese Medicine Entrepreneurship and Innovation Competition Gold Award

2021 Zhejiang University of Technology Xin'an Cup · Canal Incentive Plan

2020 National College Student Innovation and Entrepreneurship Training Program

2020 Zhejiang Province New Seedling Plan

Third Prize of the Challenge Cup in Zhejiang Province in 2020

Host and main participants:

1. 2022 Pioneer Plan digital agriculture Factory Key Technology and Industrialization Application Subproject, 2022.04 - now, presided over

2. Development and Application of Bacterial Clinical Drug Sensitivity Testing Kit, Horizontal Project, 2020.12.01-2023.11.30, Part 2

3. Application of a Bacteriocin-producing Plant Lactobacillus ZJ5 in the Treatment of Helicobacter pylori Infection, Zhejiang Provincial Public Welfare Project, 2019-2022, Third

4. Application of unlabeled Raman spectroscopy imaging technology for cytotoxicity screening of glutaminase inhibitors, Zhejiang Natural Science Foundation project, November 2019 to December 2022, led by

5. Research and application of rapid detection methods for ingredients in traditional Chinese medicine slices, horizontal project, 2019-2021, led by

6. Screening and Preparation of Monoclonal Antibodies Against Pathogenic Binding Mycobacterium, Horizontal Project, 2017.12-2022.11, Hosted

7. Study on the Mechanism and Method of Early Rapid Diagnosis of Plant Infection by Sclerotinia sclerotiorum through Spectral Imaging (1471417), General Project of the National Natural Science Foundation of China, 2015.01-2018.12, Part 2

8. Raman detection and imaging research on interaction between gene targeting small molecules and cells, Zhejiang Science and Technology Plan Project, 2014.01-2015.12, presided over

9. Raman Chemical Imaging Mechanism and Characteristics of Polysaccharides in Leaf Cell Wall Infected by Tea Cloud Leaf Blight (61201073), National Natural Science Foundation of China Youth Science Foundation Project, 2013.01-2015.12, Part 3

10. Research on the Preparation of Monoclonal Antibodies Against Tuberculosis Specific Antigen ESAT-6/CFP-10, Horizontal Project, August 2011-2014.08, Hosted

11. Research on the Role of plasma cell like Dendritic Cells in the Pathogenesis of Chronic Hepatitis B, Young Scientists Fund of the National Natural Science Foundation of China, 2009.01-2011.12, presided over

12. Research on the Application of Near Infrared Spectroscopy Technology in the Quality Inspection of Fermented Cordyceps, 2008-08-2014.08, Horizontal Project, Hosted

Publication：

 1.    Xu Y,  Hou X  L, Zhu Q Q, Mao S J, Ren J, Lin J D, Xu N*, Phenotype Identification of HeLa Cells Knockout CDK6 Gene Based on Label-free Raman Imaging, Analytical Chemistry, 2022, 94(25): 8890-8898.

2.     Mao S J, Ren J, Xu Y, Lin J D, Pan C Q, Meng Y, Xu N*, Studies in the antiviral molecular mechanisms of 25-hydroxycholesterol: Disturbing cholesterol homeostasis and post-translational modification of proteins, European Journal of Pharmacology, 2022;926:175033.

3.     Ren J, Mao S J, Lin J D, Xu Y, Zhu Q Q, Xu N*, Research Progress of Raman Spectroscopy and Raman Imaging in Pharmaceutical Analysis, Current Pharmaceutical Design, 2022.

4.      Zhu Q Q, Xu N N, Xu Y, Dong Y Y, Xu N*. Label-free Surface-enhanced Raman Spectroscopy Detection for Tyrosine-Methionine-Aspartate-Aspartate (YMDD)-motif mutants of HBV DNA[J]. Vibrational Spectroscopy, 2021, 114:103253.

5.      Xie L, Chen Z, Liu W, Gu D S, Yu Y, Chen X R, Wu Y F, Xu N, et al. A sensitive EZMTT method provides microscale, quantitative and high-throughput evaluation of drug efficacy in the treatment of Mycobacterium tuberculosis infectious diseases[J]. Journal of Microbiological Methods, 2021, 181:106136.

6.      Bian Y F, Hou W, Chen X R, Fang J Z, Xu N*, et al. Glutamate Dehydrogenase as a Promising Target for Hyperinsulinism Hyperammonemia Syndrome Therapy, Current Medicinal Chemistry, 2021.

7.      Rui J#, Xu N#, Yin J B, et al. The EZMTT cell proliferation assay provides precise measurement for drug combinations and better correlation between in vitro and in vivo efficacy[J]. Bioorganic & Medicinal Chemistry Letters, 2020, 30(11):127134.

8.      Xu N, Zhu P P, Liang J, et al. Label-free Raman spectroscopy monitoring of cytotoxic response induced by a telomerase inhibitor[J]. Sensors and Actuators B: Chemical, 2019, 293: 1-10.

9.      Liang J, Li X L, Zhu P P, Xu N*, et al Hyperspectral Reflectance Imaging Combined with Multivariate Analysis for Diagnosis of Sclerotinia Stem Rot on Arabidopsis Thaliana Leaves[J]. Applied Sciences, 2019, 9(10).

10.    Chen Z#, Li D#, Xu N#, et al. Novel 1,3,4-Selenadiazole-Containing Kidney-Type Glutaminase Inhibitors Showed Improved Cellular  Uptake and Antitumor Activity[J]. Journal of medicinal chemistry, 2019, 62(2):589-603.

11.    Xu Y, Liu L, Huang M Z, Xu N*. High accuracy determination of Angelica dahurica origin based on near infrared spectroscopy and random forest pruning algorithm[J]. Journal of Near Infrared Spectroscopy, 2019, 27(4):278-285.

12.    Xu N N, Xu N*, Liu L, et al. Minireview: Recent Advances in Surface-Enhanced Raman Scattering-Based Nucleic Acid Detection with Application to Pathogen Diagnosis[J]. Analytical Letters, 2018:1-17.

13.    Li X, Wei Y, Xu J, Xu N, et al. Quantitative visualization of lignocellulose components in transverse sections of moso bamboo based on FTIR macro- and micro-spectroscopy coupled with chemometrics[J]. Biotechnology for Biofuels, 2018, 11(1).

14.    Zhao Y R, Li X L, Xu N, et al. Application of Raman Spectroscopy Technique for Detecting the Physiological Information of Plants: A Review[J]. Spectroscopy and Spectral Analysis, 2017, 37(5): 1350-1356.

15.   Yu X L, Xu N, He Y*. Research progress in the application of near infrared analysis technology in the detection of amino acids in food[J]. Spectroscopy and Spectral Analysis. 2014(9):5. Chinese.

16.   Wei X, Xu N, Wu D, He Y. Determination of Branched-Amino Acid Content in Fermented Cordyceps sinensis Mycelium by Using FT-NIR Spectroscopy Technique[J]. Food & Bioprocess Technology February 2014, 7(1):184-190.

17.    Luo W Q, Yang H Q, Xu N. Application of near infrared spectroscopy in rapid identification of Celastrus Varieties[J]. Infrared Spectroscopy. 2013, 34(1):15-19. Chinese.

18.    Xu N, Wei X, Ren B, et al. Near-infrared spectroscopy analysis of adenosine and water in fermentation cordyceps powder and wavelength assignment[J]. Spectroscopy and Spectral Analysis. 2012,32(7):1762-5. Chinese.

19.    Xu N, Yao H P, Lv G C, et al. Downregulation of TLR7/9 leads to deficient production of IFN-α from plasmacytoid dendritic cells in chronic hepatitis B[J]. Inflamm Res. 2012;61(9):997-1004.

20.    Fang M X, Xu N, Shao X T, et al. Inhibitory effects of human immunodeficiency virus gp120 and Tat on CpG-A-induced inflammatory cytokines in plasmacytoid dendritic cells[J]. Journal of Biochemistry and Biophysics. 2012,44(9):797

21.    Xu N, Tu J J, Wang X Y, et al. Construction and High Expression of a Recombinant Amino-Proximal HSV-2 Glycoprotein B Polypeptide with Immunogenicity in Escherichia coli [J]. Letters in Drug Design & Discovery, 2012,809-814(6)

22.    Lou X E, Xu N, Yao H P, et al. Bicyclol attenuates pro-inflammatory cytokine and chemokine productions in CpG-DNA-stimulated L02 hepatocytes by inhibiting p65-NF-kappaB and p38-MAPK activation[J]. Pharmazie. 2010, 65(3):206-12.

23.    Xu N, Yao H P, Sun Z, et al. Toll-like receptor 7 and 9 expression in peripheral blood mononuclear cells from patients with chronic hepatitis B and related hepatocellular carcinoma[J]. Acta Pharmacol Sin, 2008,29(2):239-44

24.   Xu N, Yang Z G, Zhu H H*, et al. Inhibition of HBV core antigen gene expression in human embryonic kidney cell line AD293 by  plasmid-based RNAi[J]. Journal of Zhejiang University (Medicine Sciences). 2005,34(2):104-109. Chinese.

Patent：

1. Xu N, Yao H P. China patent submission for “Immunochromatographic strip for rapid detection of Mycobacterium tuberculosis secreted proteins and their preparation” (CN 10242624 A)

2. Xu N, Liu L. China patent submission for “A method for distinguishing and processing information of atractylodes granules” (CN 111398211 A)

3. Liu Y, Liu K X, Xu N, Lou W Y. China patent submission for “Quality prediction method of Angelica dahurica decoction pieces based on hyperspectral imaging depth analysis” (CN 113008805 A) 

4. Wang W J, Ruan B F, Xu N. China patent submission for “Antibiotic susceptibility and compound antibacterial activity detection method based on EZMTT reagent” (CN 111705107 A)