Tong shengqiang

Positions:

2024.12-present Director of the Institute of Traditional Chinese Medicine

2016.12 - present       Professor

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China

2011.12 - 2016.11       Associate professor

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China

2013.8 - 2014.9      Visiting scholar

National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health, Bethesda, Maryland, USA

2006.6 - 2011.11        Lecturer

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China

2004.6 - 2006.11        Teaching Assistant

College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, China

Education:

2007.9-2011.6    Ph.D. in Biochemical Engineering

Zhejiang University, Hangzhou, China

2001.9-2004.6    M.S. in Biochemical Engineering

Zhejiang University of Technology, Hangzhou, China

1996.9-2000.6    B.S. in Chemical Engineering

Zhejiang University of Technology, Hangzhou, China

2004.9-2013.8 Chemical pharmaceutical technology

2014.9-2021.8 Chemical pharmaceutical technology

2018.9-present Scientific Writing

2018.9-present Modern Instrumental Analysis

2020-present publications:

1.       Huizhen Ni, Wentao Xu, Linna Xu, Songlin Chen, Junchao Zhu, Shengqiang Tong*. Mercapto-β-Cyclodextrin Grafting Uniform Spherical Covalent Organic Frameworks for Enantioseparation in Liquid Chromatography. Analytica Chimica Acta, 2025, 1363, 344186.

2.       Junchao Zhu, Linna Xu, Wentao Xu, Huizhen Ni, Songlin Chen, Chu Chu, Dongping Cheng, Shengqiang Tong*. Multi-Interaction (Substituted-Phenylcarbamate)-Hydroxypropyl-β-Cyclodextrin Chiral Stationary Phases for Liquid Chromatography. Analytica Chimica Acta, 2025, 1358, 344104.

3.       Chenlei Ma#, Honglei Bao#, Beibei Zhu, Junchao Zhu, Songlin Chen, Huawei Lv, Chu Chu, Shengqiang Tong*. Separation of Sesquiterpene Glycosides with α-Glucosidase Inhibitory Activity from Eriobotrya japonica (Thunb.) Leaves by High-Speed Countercurrent Chromatography. Journal of Chromatography A, Available online 15 February 2025, 1746, 465780.

4.       Tao Ke, Zhongyuan Zhao, Junhuan Lin, Fengting Ou, Kaikai Chen, Kui Zeng, Debo He, Shengqiang Tong, Xinyi Wang, Lushan Yu*, Jing Chen. Development of a UHPLC-MS/MS method for the quantification of PristinamycinIA and IIA in beagle dog plasma and its pharmacokinetic application. Journal of Pharmaceutical and Biomedical Analysis, 2024, 251. 116401.

5.       Wenyu Sun, Huiyun Qiu, Susu Lin, Shengqiang Tong*. Impact of the degree of substitution on the enantioseparation of anionic β-cyclodextrin in liquid‒liquid extraction and countercurrent chromatography: Insights from molecular docking simulations. Journal of Chromatography A 1733 (2024) 465255.

6.       Huiyun Qiu, Beibei Zhu, Junchao Zhu, Songlin Chen, Chenlei Ma, Haiping Xiang, Shengqiang Tong*.Recent Advances in Application of Cyclodextrin-based Chiral Materials for Enantioseparation. TrAC Trends in Analytical Chemistry, 2024, 175: 117708.

7.       Junchao Zhu, Songlin Chen, BeiBei Zhu, Chenlei Ma, Huiyun Qiu, Lang Chen, Shengqiang Tong*. Enantiomeric analysis of γ(δ)-lactones by reversed-phase high-performance liquid chromatography using amylose tris(5-chloro-2-methylphenylcarbamate) as stationary phase. Journal of Chromatography A, Available online 29 February 2024, 1720: 464779.

8.       Songlin Chen, Junchao Zhu, Chenlei Ma, Beibei Zhu, Huiyun Qiu, Shengqiang Tong*. Mercapto-β-cyclodextrin Covalent Organic Frameworks for Enantioselective Liquid-Liquid Extraction. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2024, 687: 133496.

9.       Weiyi Wen, Haiping Xiang, Huiyun, Qiu, Jianwei Chen, Xuemin Ye*, Lin Wu, Zhiqiang Chen*, Shengqiang Tong*. Screening and identification of antibacterial components in Artemisia argyi essential oil by TLC – direct bioautography combined with comprehensive 2D GC×GC-TOFMS. Journal of Chromatography B, Available online 23 January 2024, 124026.

10.   Ping Xu, Haiping Xiang, Weiyi Wen, Sihua Quan, Huiyun Qiu, Chu Chu, Shengqiang Tong*. Application of Two-Dimensional Reversed Phase Countercurrent Chromatography×High-Performance Liquid Chromatography to Bioactivity-guided Screening and Isolation of α-Glucosidase Inhibitors from Rheum palmatum L. Journal of Chromatography A, Volume 1717, 22 February 2024, 464667.

11.   Haiping Xiang, Weiyi Wen, Ping Xu, Huiyun Qiu, Chu Chu, Qingsong Shao*, Shengqiang Tong*.Inhibition mechanism of α-glucosidase by three geranylated compounds: Kinetic, spectroscopic and molecular docking. Process Biochemistry, 2024, 136, 237-244.

12.   Sihua Quan, Mengyi Wen, Ping Xu, Chu Chu, Hui Zhang, Kai Yang*, Shengqiang Tong*. Efficient screening pancreatic lipase inhibitors from Rheum palmatum by affinity ultrafiltration-high performance liquid chromatography combined with high-resolution inhibition profiling, Phytochemical Analysis, 2024,35(3):540-551.

13.   Ben Chen, Songlin Chen, Mengyi Wen, Junchao Zhu, Shengqiang Tong*. Investigation of Three Sulfobutylether-ß-cyclodextrin Bonded Magnetic Nanoparticles in Enantioselective Liquid-Liquid Extraction, Journal of Molecular Liquids, Available online 16 October 2023, 391(Part_B), 123362.

14.   Liqun Fang, Mengyi Wen, Yanfang Zou, Chu Chu, Chunyan Wu*, Shengqiang Tong*. Matrix solid phase dispersion combined with micro-fractionation bioactivity evaluation screening polymethoxyflavones from Citrus peels. Journal of Separation Science, First published: 27 September 2023, 46(22), 2300570.

15.   Beibei Zhu, Huiyun Qiu, Chenlei Ma, Songlin Chen, Junchao Zhu, Shengqiang Tong*. Recent progress on chiral extractants for enantioselective liquid-liquid extraction. Journal of Chromatography A, Available online 17 September 2023, 1709, 464389.

16.   Tingting Lin, Beibei Zhu, Mengyi Wen, Chenlei Ma, Shengqiang Tong*.Retention correlation and orthogonality between reversed phase countercurrent chromatography and liquid chromatography based on solvent strength. Journal of Chromatography A, Available online 23 August 2023, 1707, 464322. 

17.   Shandong Mao, Di Zhang, Shengqiang Tong*, Juan Jin*, Qiang He. Protective effect on pancreatic β cells of α-glucosidase inhibitors screened from Rehmannia glutinosa, Food Bioscience, 2023, Available online 11 August 2023, 55, 103022.

18.   Huiyun Qiu, Haiping Xiang, Mengyi Wen, Songlin Chen, Junchao Zhu, Shengqiang Tong*.Enantioseparation of two antifungal azole drugs by analytical countercurrent chromatography using sulfobutyl ether-β-cyclodextrin as chiral selector. Journal of Chromatography A, 2023, Available online 29 June 2023, 1705, 464185.

19.   Haiping Xiang, Ping Xu, Weiyi Wen, Huiyun Qiu, Chu Chu*, Qingsong Shao*, Shengqiang Tong*. Screening, characterization of trace α-glucosidase inhibitors from the root of Pueraria lobata and evaluation of their hypoglycemic activity. Food Bioscience, 2023, 53: 102641. Available online 11 April 2023, 53, 102641.

20.   Mengyi Wen, Sihua Quan, Huiyun Qiu, Weiyi Wen, Ben Chen,Yuxia Chen*, Jianyun Peng*, Shengqiang Tong*. High-resolution monophenolase/diphenolase/radical scavenging profiling for rapid screening of natural whitening candidates from Rosa rugosa cv. ‘Plena’. Journal of Separation Science, 2023, 46(13), 2200987.

21.   Liqun Fang, Tingting Lin, Ben Chen, Haibo You, Chunyan Wu, Chu Chu, Shengqiang Tong*. HPLC micro-fraction bioactive evaluation combined with countercurrent chromatographic separation of antioxidants from Citrus peel and their tyrosinase inhibition activities. Journal of Separation Science, 2023, 46(4), 2200764.

22.   Wenyu Sun, Huiyun Qiu, Haibo You, Ben Chen, Liqun Fang, Junqing Qian, Shengqiang Tong*. Degree and distribution of substitution of hydroxypropyl-β-cyclodextrin in enantioselective liquid-liquid extraction and countercurrent chromatographic enantioseparation. Journal of Chromatography A, 2023, 1687, 463684.

23.   Weiyi Wen, Ping Xu, Haiping Xiang, Mengyi Wen, Xuemin Ye, Chu Chu*, Shengqiang Tong*. Comprehensive two-dimensional countercurrent chromatography × gas chromatography characterization of Artemisia argyi essential oil, Analytica Chimica Acta, 2023, 1237, 340614.  

24.   Haibo You, Ben Chen, Liqun Fang, Tingting Lin, Ping Xu, Chu Chu, Shengqiang Tong*. Analytical enantioseparation of N-alkyl drugs by reversed phase liquid chromatography with carboxymethyl-β-Cyclodextrin as mobile phase additive. Chirality, 2023, 35(1), 58-66.

25.   Xiaoyuan Lv, Haibo You,Huiyun Qiu, Mengyi Wen,Chuncui Zheng, Shengqiang Tong*. Separation of Acetylferrocene from synthetic mixtures by countercurrent chromatography. Journal of Liquid Chromatography & Related Technologies, 2022, 45(5-8), 94-99.

26.   Ping Xu, Xiang Wang, Tingting Lin, Qingsong Shao, Jianyun Peng, Chu Chu, Shengqiang Tong*.A strategy for pinpointing natural bioactive components using 2D bioassay profilings combined with comprehensive 2D CCC×HPLC. Analytical Chemistry, Publication Date: September 8, 2022, 94(37): 12715-12722.

27.   Tingting Lin; Ben Chen; Liqun Fang; Haibo You; Chu Chu; Qingsong Shao; Shengqiang Tong*. Solvent strength of organic phase for two biphasic solvent systems in high speed countercurrent chromatography, Journal of Chromatography A, Available online 14 August 2022, 1680: 463422.

28.   Heng-Mian Sun, Ai-Lian Zhang, Hong-Lei Bao, ChuChu*, Sheng-Qiang Tong*, In Silico Screening of Off-line Comprehensive Two-Dimensional Counter-current Chromatography with Liquid Chromatography for Four Saponins Isolation. Journal of Separation Science, 2022, 45(20), 3909-3918.

29.   Ben Chen, Tingting Lin, Haibo You, Liqun Fang, Chu Chu, Jintao Yang*, Shengqiang Tong*. Preparation of Sulfobutylether-β-cyclodextrin Bonded Fe3O4/SiO2 Core-Shell Nanoparticles and its Application in Enantioselective Liquid-Liquid Extraction. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2022, 652: 129861.

30.   Haiping Xiang, Ping Xu, Huiyun Qiu, Weiyi Wen, Ailian Zhang, Shengqiang Tong*. Two-dimensional chromatography in screening of bioactive components from natural products, Phytochemical Analysis, 2022, 33(8), 1161-1176.

31.   Wenyu Sun, Ben Chen, Haibo You, Liqun Fang, Junqing Qian*, Shengqiang Tong*. Enantioseparation of N-methyl duloxetine, duloxetine and fluoxetine by countercurrent chromatography using anionic β-cyclodextrin as chiral selector,Journal of Separation Science, First published: 22 May 2022, 45(15), 3022-3030.

32.   Ben Chen, Haibo You, Liqun Fang, Tingting Lin, Ping Xu, Chu Chu, Shengqiang Tong*. Enantioseparation of five racemic N-alkyl drugs by reverse phase HPLC using sulfobutylether-β-cyclodextrin as a chiral mobile phase additive, Journal of Separation Science, First published: 24 March 2022,45(11),1847-1855.

33.   Tingting Lin, Ping Xu, Ben Chen, Liqun Fang, Haibo You, Shengqiang Tong*, Solvent strength of aqueous phase for two typical biphasic solvent systems in high-speed countercurrent chromatography, Journal of Chromatography A, 2022, 1663, 462767.

34.   Honglei Bao, Wenyu Sun, Hengmian Sun, Yang Jin, Xingchu Gong, Chu Chu, Shengqiang Tong*. Liquid chromatographic study of two structural isomeric pentacyclic triterpenes on reversed-phase stationary phase with hydroxypropyl-β-cyclodextrin as mobile phase additive，Journal of Pharmaceutical and Biomedical Analysis，2022, 207, 114420.

35.   Hengmian Sun, Li-Juan Ma, Jian-Bo Wan*, Shengqiang Tong*. Preparative separation of gypenoside XVII, ginsenoside Rd2, and notoginsenosides Fe and Fd from Panax notoginseng leaves by countercurrent chromatography and orthogonality evaluation for their separation. Journal of separation science,  2021, 44(15), 2996-3003.

36.   Honglei Bao, Wenyu Sun, Yang Jin, Chaoyue Wang, Xiang Wang, Hengmian Sun, Chu Chu*, Shengqiang Tong*,Preparative separation of structural isomeric pentacyclic triterpenes from Eriobotrya japonica (Thunb.) leaves by high speed countercurrent chromatography with hydroxypropyl-β-cyclodextrin as additive, Journal of Chromatography A, 2021, 1646, 462066. 

37.   Shanshan Zhao, Xiang Wang, Wenyu Sun, Xingchu Gong, Jizhong Yan, Shengqiang Tong*. Application of Liquid-liquid chromatography as a sample pretreatment method for quantitative analysis of synephrine in Fructus Aurantii Immaturus. Journal of Liquid Chromatography & Related Technologies, 2021, 44 (3-4): 189-196.

38.   Yang Jin, Honglei Bao, Wenyu Sun, Shengqiang Tong*. Enantioseparation of three constitutional isomeric 2-(methylphenyl)propanoic acids by countercurrent chromatography,Journal of Chromatography A, 2021, 1637: 461804.

39.   Wenyu Sun, Honglei Bao, Junqing Qian*, Shengqiang Tong*. Enantioseparation of ondansetron by countercurrent chromatography using sulfobutyl ether-β-cyclodextrin as chiral selector, Journal of separation science, 2021, 44: 922–930.

40.   Yang Jin, Honglei Bao, Wenyu Sun, Hengmian Sun, Shanshan Zhao, Xiang Wang, Shengqiang Tong*. Enantioseparation of 2-(4-chlorophenyl)succinic acid by countercurrent chromatography and investigation of injection volume on resolution, Journal of separation science,2021, 44: 752–758.

41.   Xiang Wang, Shanshan Zhao, Chaoyue Wang, Wei Du, Hengmian Sun, Wenyu Sun, Yang Jin, Guanglei Zuo, Shengqiang Tong*. Orthogonality in the selection of biphasic solvent systems for off-line two-dimensionalcountercurrent chromatography from Polygonum cuspidatum Sieb. et Zucc. Journal of Chromatography A, 2020, 1634: 461666.

42.   Yang Jin, Wenyu Sun, Huawei Lv, Shengqiang Tong*. Spectral study on inclusion interaction and enantiorecognition of 2-aryl carboxylic acids with hydroxypropyl-β-cyclodextrin, Chirality, 2020, 32, 1257–1263.

43.   Chaoyue Wang, Guanglei Zuo, Xiang Wang, Hyun Yong Kim, Shanshan Zhao,Wenyu Sun, Shengqiang Tong*, and Soon Sung Lim*. Retention mechanism of pH-peak-focusing in countercurrent chromatographic separation of baicalin and wogonoside from Scutellaria baicalensis Georgi. Journal of Separation Science, 2020, 43(19), 3806-3815.

44.   Xiang Wang, Guang-Lei Zuo, Chao-Yue Wang, Hyun Yong Kim, Soon Sung Lim and Sheng-Qiang Tong*. An Off-Line DPPH-GC-MS Coupling Countercurrent Chromatography Method for Screening, Identification, and Separation of Antioxidant Compounds in Essential Oil, Antioxidants 2020, 9, 702.

45.   Chaoyue Wang, Xiang Wang, Shanshan Zhao, Guanglei Zuo, Min Xu, Shengqiang Tong, Liquid chromatographic and liquid-liquid chromatographic separation of structural isomeric oleanolic acid and ursolic acid using hydroxypropyl-β-cyclodextrin as additive, Journal of Chromatography A, 2020, 1625, 461332.

46.   Shengqiang Tong, Liquid-liquid chromatography in enantioseparations, Journal of Chromatography A, 2020, 1626, 461345.

47.   Shanshan Zhao, Chaoyue Wang, Xiang Wang, Yang Jin, Wenyu Sun, Xingchu Gong, Shengqiang Tong. Liquid-liquid chromatography in sample pretreatment for quantitative analysis of trace component in traditional Chinese medicines by conventional liquid chromatography, Journal of Chromatography A, 2020, 1619, 460917.

48.   Wenyu Sun, Yang Jin, Chaoyue Wang, Shanshan Zhao, Xiang Wang, Meng Luo, Jizhong Yan, Shengqiang Tong. Stereoselective separation of isomeric sertraline with analytical countercurrent chromatography, Journal of Chromatography A 2020, 1617, 460834.

49.   Huawei Lv, Mengxia Lu, Shengqiang Tong*. Chromatographic study of four sesquiterpenoids in volatile oil of Curcumae Rhizoma on reverse phase stationary phase with methyl-β-cyclodextrin as mobile additive, Journal of Liquid Chromatography & Related Technologies, 2020, 43(13-14), 508-515.

50.   Xujun Qiu, Wenyu Sun, Chaoyue Wang, Jizhong Yan, Shengqiang Tong*. Enantioseparation of acetyltropic acid by countercurrent chromatography with sulfobutyl ether-β-cyclodextrin as chiral selector, Journal of separation science, 2020, 43:681–688.

Screening of natural bioactive components

Preparation of chiral stationary phase for HPLC 

Chinese medicine quality control based on bioactivity

Enantioselective liquid-liquid extractions and countercurrent chromatography

Comprehensive two-dimensional chromatography